Journal: Journal of Clinical Microbiology

Article Title: Analytical and Clinical Comparison of Three Nucleic Acid Amplification Tests for SARS-CoV-2 Detection

doi: 10.1128/JCM.01134-20

Figure Lengend Snippet: Basic assay characteristics and workflow parameters of three EUA SARS-CoV-2 NAATs ^a

Article Snippet: The BioFire COVID-19 test (BioFire) (BioFire Defense, Salt Lake City, UT) was performed on the BioFire FilmArray Torch 12-bay system according to the manufacturer’s instructions.

Techniques: Virus

Journal: Expert Review of Molecular Diagnostics

Article Title: Critical evaluation of FDA-approved respiratory multiplex assays for public health surveillance

doi: 10.1080/14737159.2018.1487294

Figure Lengend Snippet: Comparison of sensitivity (Sen), specificity (Spe), and diagnostic odds ratio (DOR, with 95%CI) of FDA-approved respiratory panels, based on data reported by manufacturers to the FDA as part of the approval processes for the assays. For the purposes of surveillance studies, assays with larger DORs have better accuracy for the pathogen(s) of interest.

Article Snippet: In early 2016, BioFire Diagnostics (now a subsidiary of BioMerieux) received FDA clearance for an expanded instrument system, the FilmArray Torch.

Techniques: Diagnostic Assay, Luminex

Journal: Expert Review of Molecular Diagnostics

Article Title: Critical evaluation of FDA-approved respiratory multiplex assays for public health surveillance

doi: 10.1080/14737159.2018.1487294

Figure Lengend Snippet: List of all direct comparative studies of the major FDA-approved multiplex respiratory assays.

Article Snippet: In early 2016, BioFire Diagnostics (now a subsidiary of BioMerieux) received FDA clearance for an expanded instrument system, the FilmArray Torch.

Techniques: Multiplex Assay, Luminex

Journal: European Journal of Immunology

Article Title: Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections

doi: 10.1002/eji.202048978

Figure Lengend Snippet: Type‐I Interferon quantification using FilmArray ® platform. (A) Spearman correlation [IC95%] between ISG score as defined by a 6‐interferon stimulated gene transcriptional signature using Nanostring ® platform and ISG score as defined by a 4‐interferon stimulated gene transcriptional signature using FilmArray ® technology, obtained from whole blood from (i) febrile infants (n = 86 patients from one experiment, green circle) with proven infections attending pediatric emergency departments, (ii) patients with severe infection caused by SARS‐CoV2 hospitalized in the ICU (n = 26 patients from one experiment, red square), and (iii) healthcare workers with COVID‐19 with mild symptoms (n = 7 patients from one experiment, blue triangle) (ρ = 0.991). (B) Spearman correlation between ISG score as defined by a 4‐interferon stimulated gene transcriptional signature using FilmArray ® technology and IFN‐α2 concentrations measured by SIMOA ® platform, obtained from whole blood from (i) febrile infants (n = 56 patients from one experiment, green circle) with proven infections attending pediatric emergency departments, (ii) patients with severe infection caused by SARS‐CoV2 hospitalized in the ICU (n = 16 patients from one experiment, red square), and (iii) healthcare workers with COVID‐19 with mild symptoms (n = 7 patients from one experiment, blue triangle) (ρ = 0.832). Patients are represented by different forms and nonlinear regression (second order polynomial, quadratic) is represented by a black line. IFN (Interferon), ISG (Interferon Stimulated Genes).

Article Snippet: The PAXgene TM blood samples were mixed with 800 μL of the Sample Buffer provided with the kit and directly injected into the pouch and ran on FilmArray ® 2.0 and FilmArray ® Torch instruments (BioFire Diagnostics, LLC., Salt Lake City, UT).

Techniques: Infection

Journal: European Journal of Immunology

Article Title: Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections

doi: 10.1002/eji.202048978

Figure Lengend Snippet: Type‐I IFN assessment in COVID‐19 critically ill patients using FilmArray ® platform. ISG score as defined by a 4 interferon stimulated gene transcriptional signature was determined by IFN pouch FilmArray ® panel, obtained from whole blood from selected patients severely infected by SARS‐CoV‐2 at ±5 days after admission in the ICU (n = 16 patients from one experiment) and healthy volunteers (n = 10 donors from one experiment). COVID‐19 critically‐ill patients are represented by a black dot, and these samples are stratified according to their impairment of IFN response. The healthy volunteers (n = 10 donors from one experiment) are represented by a black dot also. The dashed line represents the arbitrary threshold of the ISG score set to separate patients with a positive (n = 4 patients from one experiment) or negative (n = 12 patients from one experiment) IFN response. The box‐and‐whisker plots represent the median and min‐max; Mann–Whitney U test ** p < 0.01; **** p < 0.0001. IFN (Interferon), ISG (Interferon Stimulated Genes).

Article Snippet: The PAXgene TM blood samples were mixed with 800 μL of the Sample Buffer provided with the kit and directly injected into the pouch and ran on FilmArray ® 2.0 and FilmArray ® Torch instruments (BioFire Diagnostics, LLC., Salt Lake City, UT).

Techniques: Infection, Whisker Assay, MANN-WHITNEY